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Ecological signals of arctic plant-microbe associations are consistent across eDNA and vegetation surveys

Publiceringsår

2023

Upphovspersoner

Parisy, Bastien; Schmidt, Niels M.; Wirta, Helena; Stewart, Laerke; Pellissier, Loic; Holben, William E.; Pannoni, Sam; Somervuo, Panu; Jones, Mirkka, M.; Siren, Jukka; Vesterinen, Eero; Ovaskainen, Otso; Roslin, Tomas

Abstrakt

Understanding how different taxa respond to abiotic characteristics of the environment is of key interest for understanding the assembly of communities. Yet, whether eDNA data will suffice to accurately capture environmental imprints has been the topic of some debate. In this study, we characterised patterns of species occurrences and co-occurrences in Zackenberg in northeast Greenland using environmental DNA. To explore the potential for extracting ecological signals from eDNA data alone, we compared two approaches (visual vegetation surveys and soil eDNA metabarcoding) to describing plant communities and their responses to abiotic conditions. We then examined plant associations with microbes using a joint species distribution model. We found that most (68%) of plant genera were detectable by both vegetation surveys and eDNA signatures. Species-specific occurrence data revealed how plants, bacteria and fungi responded to their abiotic environment - with plants, bacteria and fungi all responding similarly to soil moisture. Nonetheless, a large proportion of fungi decreased in occurrences with increasing soil temperature. Regarding biotic associations, the nature and proportion of the plant-microbe associations detected were consistent between plant data identified via vegetation surveys and eDNA. Of pairs of plants and microbe genera showing statistically supported associations (while accounting for joint responses to the environment), plants and bacteria mainly showed negative associations, whereas plants and fungi mainly showed positive associations. Ample ecological signals detected by both vegetation surveys and by eDNA-based methods and a general correspondence in biotic associations inferred by both methods, suggested that purely eDNA-based approaches constitute a promising and easily applicable tool for studying plant-soil microbial associations in the Arctic and elsewhere.
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Organisationer och upphovspersoner

Åbo universitet

Vesterinen Eero

Jyväskylä universitet

Ovaskainen Otso Orcid -palvelun logo

Helsingfors universitet

Parisy Bastien

Wirta Helena

Siren Jukka

Jones Mirkka M.

Ovaskainen Otso

Somervuo Panu

Roslin Tomas

Helsingforsregionens universitetscentralsjukhus specialupptagningsområde

Parisy Bastien

Vesterinen Eero

Wirta Helena

Siren Jukka

Jones Mirkka M.

Ovaskainen Otso

Somervuo Panu

Roslin Tomas

Publikationstyp

Publikationsform

Artikel

Moderpublikationens typ

Tidning

Artikelstyp

En originalartikel

Målgrupp

Vetenskaplig

Kollegialt utvärderad

Kollegialt utvärderad

UKM:s publikationstyp

A1 Originalartikel i en vetenskaplig tidskrift

Publikationskanalens uppgifter

Moderpublikationens namn

Metabarcoding and Metagenomics

Volym

7

Artikelnummer

e99979

Sidor

155-197

Publikationsforum

86802

Publikationsforumsnivå

1

Öppen tillgång

Öppen tillgänglighet i förläggarens tjänst

Ja

Öppen tillgång till publikationskanalen

Helt öppen publikationskanal

Parallellsparad

Ja

Övriga uppgifter

Vetenskapsområden

Miljövetenskap; Ekologi, evolutionsbiologi; Växtbiologi, mikrobiologi, virologi

Nyckelord

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Publiceringsland

Bulgarien

Förlagets internationalitet

Internationell

Språk

engelska

Internationell sampublikation

Ja

Sampublikation med ett företag

Nej

DOI

10.3897/mbmg.7.99979

Publikationen ingår i undervisnings- och kulturministeriets datainsamling

Ja