Ecological signals of arctic plant-microbe associations are consistent across eDNA and vegetation surveys
Publiceringsår
2023
Upphovspersoner
Parisy, Bastien; Schmidt, Niels M.; Wirta, Helena; Stewart, Laerke; Pellissier, Loic; Holben, William E.; Pannoni, Sam; Somervuo, Panu; Jones, Mirkka, M.; Siren, Jukka; Vesterinen, Eero; Ovaskainen, Otso; Roslin, Tomas
Abstrakt
Understanding how different taxa respond to abiotic characteristics of the environment is of key interest for understanding the assembly of communities. Yet, whether eDNA data will suffice to accurately capture environmental imprints has been the topic of some debate. In this study, we characterised patterns of species occurrences and co-occurrences in Zackenberg in northeast Greenland using environmental DNA. To explore the potential for extracting ecological signals from eDNA data alone, we compared two approaches (visual vegetation surveys and soil eDNA metabarcoding) to describing plant communities and their responses to abiotic conditions. We then examined plant associations with microbes using a joint species distribution model. We found that most (68%) of plant genera were detectable by both vegetation surveys and eDNA signatures. Species-specific occurrence data revealed how plants, bacteria and fungi responded to their abiotic environment - with plants, bacteria and fungi all responding similarly to soil moisture. Nonetheless, a large proportion of fungi decreased in occurrences with increasing soil temperature. Regarding biotic associations, the nature and proportion of the plant-microbe associations detected were consistent between plant data identified via vegetation surveys and eDNA. Of pairs of plants and microbe genera showing statistically supported associations (while accounting for joint responses to the environment), plants and bacteria mainly showed negative associations, whereas plants and fungi mainly showed positive associations. Ample ecological signals detected by both vegetation surveys and by eDNA-based methods and a general correspondence in biotic associations inferred by both methods, suggested that purely eDNA-based approaches constitute a promising and easily applicable tool for studying plant-soil microbial associations in the Arctic and elsewhere.
Visa merOrganisationer och upphovspersoner
Åbo universitet
Vesterinen Eero
Helsingfors universitet
Parisy Bastien
Wirta Helena
Siren Jukka
Jones Mirkka M.
Ovaskainen Otso
Somervuo Panu
Roslin Tomas
Helsingforsregionens universitetscentralsjukhus specialupptagningsområde
Parisy Bastien
Vesterinen Eero
Wirta Helena
Siren Jukka
Jones Mirkka M.
Ovaskainen Otso
Somervuo Panu
Roslin Tomas
Publikationstyp
Publikationsform
Artikel
Moderpublikationens typ
Tidning
Artikelstyp
En originalartikel
Målgrupp
VetenskapligKollegialt utvärderad
Kollegialt utvärderadUKM:s publikationstyp
A1 Originalartikel i en vetenskaplig tidskriftPublikationskanalens uppgifter
Journal
Moderpublikationens namn
Förläggare
Volym
7
Artikelnummer
e99979
Sidor
155-197
ISSN
Publikationsforum
Publikationsforumsnivå
1
Öppen tillgång
Öppen tillgänglighet i förläggarens tjänst
Ja
Öppen tillgång till publikationskanalen
Helt öppen publikationskanal
Parallellsparad
Ja
Övriga uppgifter
Vetenskapsområden
Miljövetenskap; Ekologi, evolutionsbiologi; Växtbiologi, mikrobiologi, virologi
Nyckelord
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Publiceringsland
Bulgarien
Förlagets internationalitet
Internationell
Språk
engelska
Internationell sampublikation
Ja
Sampublikation med ett företag
Nej
DOI
10.3897/mbmg.7.99979
Publikationen ingår i undervisnings- och kulturministeriets datainsamling
Ja