2025

Ragab, Mona S.; Shoukry, Mohamed M.; El-Madany, Hanaa M.; Haukka, Matti; van Eldik, Rudi; Khalf-Alla, Perihan A.

Novel palladium complexes, [Pd(hydten)Cl2] (1) and [Pd(hydten)ox] (2), where hydten = N-(2-hydroxyethyl) ethylenediamine and ox = oxalate, were synthesized. The structure is confirmed using X-ray crystallography for (1) and different physicochemical techniques. A comprehensive analysis was conducted to investigate the composition, stability constants, and speciation curves of [Pd(hydten)(H2O)2]2+ complexes with some bio-ligands such as amino acids, peptides, and DNA constituents. This analysis aims to gain a deeper understanding of the distribution of different species in physiological environments. The interaction with DNA, one of the primary targets for chemotherapeutic drugs, was validated using the UV-Vis spectroscopic technique combined with molecular docking analysis. The geometrical optimization of each compound was performed by density functional theory (DFT), with the complete allocation of HOMO/LUMO electron clouds. In vitro, bio-evaluating the anticancer potency of each compound was accomplished on colon cancer (HCT116), bladder cancer (T24), liver cancer (HepG2), lung cancer (A549) cancer cell lines as well as non-malignant lung cell (WI-38). The [Pd(hydten)Cl2] exhibits enhanced selective cytotoxicity contra the HCT116 malignant cell line (IC50 = 13.87 ± 3.72 μM), lower than that of 5-fluorouracil (5-FU, IC50=23.4 ± 2.4 μM), with minimal effects on healthy WI-38 cells.