This repository contains a StarDist deep learning model and its training and validation datasets for segmenting endothelial nuclei while ignoring cancer cells. The cancer cells were perfused over an endothelial cell monolayer. The initial dataset consisted of 17 images, where cancer cell nuclei were manually removed after segmentation with the StarDist Versatile Nuclei model. This dataset was augmented to 68 paired images using computational techniques like rotation and flipping. The model was trained for 200 epochs, achieving an average F1 Score of 0.976, demonstrating high accuracy in segmenting endothelial nuclei while excluding cancer cells. Specifications Model: StarDist for segmenting endothelial nuclei while ignoring cancer cells Training Dataset: Number of Original Images: 17 paired predictions of nuclei and label images Augmented Dataset: Expanded to 68 paired images using rotation and flipping Source Image Generation: Generated using a pix2pix model trained to predict nuclei from brightfield images of cancer cells on top of an endothelium (DOI: 10.5281/zenodo.10617532) Target Image Generation: Masks obtained via manual segmentation File Format: TIFF (.tif) Brightfield Images: 8-bit Masks: 8-bit Image Size: 1024 x 1022 pixels (uncalibrated) Training Parameters: Epochs: 200 Patch Size: 1024 x 1024 pixels Batch Size: 2 Performance: Average F1 Score: 0.976 Average IoU: 0.927 Model Training: Conducted using ZeroCostDL4Mic (https://github.com/HenriquesLab/ZeroCostDL4Mic/wiki) Reference Biorxiv paper

2024