Reference databases for metagenomic NGS analysis. Project repository: https://bitbucket.org/plyusnin/lazypipe/src/master/ Project website: https://www2.helsinki.fi/en/projects/lazypipe NCBI NT: Database: Minimap index for NCBI NT archaea, bacteria and viruses. 17'673'705 sequences, 392'552'172'949 bp. File: minimap.nt.abv.2023_05_16.tar.gz Database: Minimap index for NCBI NT viruses. 9'326'301 sequences, 194'755'318'770 bp. File: minimap.nt.vi.2023_06_16.tar.gz Database: BLASTN index for NCBI NT archaea and bacteria. 8'347'404 sequences, 197'796'854'179 bp. File: blast.nt.ab.2023_05_16.tar.gz Database: BLASTN index for NCBI NT viruses. 9,326,301 sequences, 194,755,318,770 bp. File: blast.nt.vi.2023_05_16.tar.gz RefSeq: Database: Minimap index for RefSeq archaea (complete, reference/representative genomes), bacteria (complete, reference/representative genomes) and viruses. 27'160 sequences, 20'170'978'762 bp. File: minimap.refseq.release219.v2.tar.gz Database: Minimap index for RefSeq archaea, bacteria (complete genomes) and viruses. 202'371 sequences, 150'389'307'001 bp. File: minimap.refseq.release219.tar.gz Database: Minimap index for RefSeq viruses. 18'689 sequences, 551'600'706 bp. File: minimap.refseq.release219.tar.gz Database: BLASTN index for RefSeq archaea and bacteria (complete, reference/representative genomes). 8,472 sequences, 19,619,389,195 bp. File: blast.refseq.ab.release219.v2.tar.gz Database: BLASTN index for RefSeq archaea and bacteria. 183,682 sequences, 149,837,706,295 bp. File: blast.refseq.ab.release219.tar.gz Database: BLASTN index for RefSeq viruses. 18,689 sequences, 551,600,706 bp. File: blast.refseq.vi.release2019.tar.gz UniRef100: Database: BLASTP index for UniRef100 archaea and bacteria. 148,440,747 sequences, 47,899,033,773 bp. File: blast.uniref100.ab.2023_06_28.tar.gz Database: BLASTP index for UniRef100 viruses. 3,034,688 sequences and 1,132,717,918 bp. File: blast.uniref100.vi.2023_06_28.tar.gz Citing: Plyusnin, Ilya, Olli Vapalahti, Tarja Sironen, Ravi Kant, and Teemu Smura. “Enhanced Viral Metagenomics with Lazypipe 2.” Viruses 15, no. 2 (February 4, 2023): 431. https://doi.org/10.3390/v15020431.

2023