The data set consists of 81 registered whole slide image pairs, a pair represents unstained and H&E stained images of the same tissue sample. In addition to that, it also contains a tissue mask for each whole slide image pair. The samples are used for studying the histological feasibility of AI-driven virtual histopathology staining. Imaging was performed using Thunder Imager 3D Tissue slide scanner (Leica Microsystems, Wetzlar, Germany) equipped with DMC2900 camera and HC PL APO 40x/0.95 DRY objective with an isotropic pixel resolution of 0.353 µm.

2022