Vertical stratification patterns of methanotrophs and their genetic controllers in water columns of oxygen-stratified boreal lakes
Publiceringsår
2021
Upphovspersoner
Rissanen, Antti J.; Saarela, Taija; Jäntti, Helena; Buck, Moritz ; Peura, Sari; Aalto, Sanni L.; Ojala, Anne; Pumpanen, Jukka; Tiirola, Marja; Elvert, Marcus; Nykänen, Hannu
Abstrakt
The vertical structuring of methanotrophic communities and its genetic controllers remain understudied in the water columns of oxygen-stratified lakes. Therefore, we used 16S rRNA gene sequencing to study the vertical stratification patterns of methanotrophs in two boreal lakes, Lake Kuivajärvi and Lake Lovojärvi. Furthermore, metagenomic analyses were done to assess the genomic characteristics of methanotrophs in Lovojärvi and a previously studied Lake Alinen Mustajärvi. The methanotroph communities were vertically structured along the oxygen gradient. Alphaproteobacterial methanotrophs preferred oxic water layers, while Methylococcales methanotrophs, consisting of putative novel genera and species, thrived especially at and below the oxic-anoxic interface and showed distinct depth variation patterns, which were not completely predictable by their taxonomic classification. Instead, genomic differences among Methylococcales methanotrophs explained their variable vertical depth patterns. Genes in COG categories L (Replication, recombination and repair) and S (Function unknown) were relatively high in metagenome-assembled-genomes representing Methylococcales thriving clearly below the oxic-anoxic interface, suggesting genetic adaptations for increased stress tolerance enabling living in the hypoxic/anoxic conditions. In contrast, genes in COG category N (Cell motility) were relatively high in metagenome-assembled-genomes of Methylococcales thriving at the oxic-anoxic interface, which suggests genetic adaptations for increased motility at the vertically fluctuating oxic-anoxic interface.
Visa merOrganisationer och upphovspersoner
Östra Finlands universitet
Nykänen Hannu
Jäntti Helena
Pumpanen Jukka
Aalto Sanni-Leea
Saarela Taija
Publikationstyp
Publikationsform
Artikel
Moderpublikationens typ
Tidning
Artikelstyp
En originalartikel
Målgrupp
VetenskapligKollegialt utvärderad
Kollegialt utvärderadUKM:s publikationstyp
A1 Originalartikel i en vetenskaplig tidskriftPublikationskanalens uppgifter
Journal
Moderpublikationens namn
Volym
97
Nummer
2
Artikelnummer
fiaa252
ISSN
Publikationsforum
Publikationsforumsnivå
1
Öppen tillgång
Öppen tillgänglighet i förläggarens tjänst
Ja
Öppen tillgång till publikationskanalen
Delvis öppen publikationskanal
Parallellsparad
Ja
Övriga uppgifter
Vetenskapsområden
Miljövetenskap; Ekologi, evolutionsbiologi; Biokemi, cell- och molekylärbiologi; Växtbiologi, mikrobiologi, virologi; Miljöteknik
Nyckelord
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Publiceringsland
Nederländerna
Förlagets internationalitet
Internationell
Språk
engelska
Internationell sampublikation
Ja
Sampublikation med ett företag
Nej
DOI
10.1093/femsec/fiaa252
Publikationen ingår i undervisnings- och kulturministeriets datainsamling
Ja